Any step flush to the top can be done out of sequence (in advance).
Any step flush left must have all of the above steps complete to succeed.
Please note that every chat is not detailed here. Use the walkthrough for full details. This is intended to be used as a checklist only.

North Freeport Elder Spirit Chat

Lake Rathe Ancient Megalodon Lustrous Seaweed Loot/Hand-in

Fungus Grove Shuddering Fungus 2x Bleak Fungus Loot/Hail

Twilight Sea Ground Spawn 3x Twinkling Shrub Loot/Hand-in

Cobalt Scar Gnashing Shark Burned Brittle Bark Loot

Shadeweaver's Thicket Wunshi Kashra 1000 Plat Hand-in

The Warrens Wilslik Gissu Chat

Plane of Justice Punisher Veshtaq Blessed Talisman of Patience Loot

Crypt of Nadox Spiritseeker Nadox Ring of The Undead Spiritchamer Loot

Dranik's Scar Ukun Gutfeaster* 3x Perfect Marked Ukun Skin Loot *or other named Ukuns

Ruins of Lxanvom Elite Corrupted Knight Corruption Powder Loot

The Grey a sun revenant 4x Revenant Tooth Loot

Howling Stones pulsating bile 2x Corrosive Goo Loot

The Warrens Thatched Medicine Bag Combine (Alchemy at 100+) & Hand-in

North Freeport Distilled Potion of Endurance Hand-in

Plane of Sky Potion of Sustenance Hand-in

North Freeport Blessed Talismans of Patience & Wisdom Hand-ins

Shadeweaver's Thicket Wunshi Kashra Chat

Umbral Planes Netherbian Swarmfiend Amulet of the Spiritist

Shadeweaver's Thicket Ring of the Undead Spiritcharmer and Amulet of the Spiritist Hand-ins

North Freeport Elder Spirit Chat

Lake Rathe Gemmed Bangle of Enlightenment Hand-in

Lake Rathe Heyokah Medicine Bag Combine & Hand-in

North Freeport Runed Purse of the McShannels Hand-in

Text-based checklist courtesy of Glaukin

Part 1: [_] - Speak to the elder spirit in North Freeport [_] - Twine-wrapped Lustrous Seaweed from Lake Rathe [_] - 2 x Bleak Fungus from Fungus Grove [_] - Tied Twinkling shrub from Twilight Sea [_] - Brittle Burned Bark from Cobalt Scar [_] - Thatched Medicine Bag from Shadeweavers Thicket [_] - Destilled Potion of Endurance from The Warrens Part 2: [_] - Hand Destilled Potion of Endurance to the elder spirit in North Freeport [_] - Blessed Talisman of Patience from Plane of Justice [_] - Blessed Talisman of Wisdom from Plane of Sky [_] - Hand talismans to elder spirit in North Freeport Part 3: [_] - Speak to Wunshi in Shadeweavers Thicket [_] - Ring of the Undead Spiritcharmer from Crypt of Nadox [_] - Amulet of the Spiritist from Umbral Plains [_] - Hand ring and amulet to Wunshi in Shadeweavers Thicket [_] - Gemmed Bangle of Enlightenment from North Freeport Part 4: [_] - Heyokah Medicine Bag from Lake Rathe [_] - 3 x Perfectly Marked Ukun Skin from Dranik's Scar [_] - Corruption Powder from Crypt of Decay [_] - 4 x Revenant Tooth from The Grey [_] - 2 x Corrosive Goo from Howling Stones [_] - Runed Purse of the McShannels from Lake Rathe [_] - Crafted Talisman of Fates from North Freeport (epic 1.5)